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mitochondrial outer membrane 20  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mitochondrial outer membrane 20
    Mitochondrial Outer Membrane 20, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 752 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mitochondrial+outer+membrane+20/Tom20+Rabbit+mAb/pmc12098161-130-59-66
    Average 97 stars, based on 752 article reviews
    mitochondrial outer membrane 20 - by Bioz Stars, 2026-09
    97/100 stars

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    Membrane:

    Article Title: TJ0113-induced mitophagy in acute liver failure detected by Raman microspectroscopy
    Article Snippet: .. Frozen liver tissue sections were fixed with 4 % polyformaldehyde (PFA), permeabilized with 0.3 % Triton X-100 for 20 min, and then blocked with 5 % BSA for 1 h. The primary antibodies used in this study included: MCL1 apoptosis regulator (Mcl-1, Cat# ab32087, Abcam), microtubule associated Protein 1 Light Chain 3 Alpha/Beta (LC3A/B, Cat# 4108, Cell Signaling Technology), mitochondrial outer membrane 20 (TOM20, Cat# 42406, Cell Signaling Technology), NLR family pyrin domain containing 3 (NLRP3, Cat# ab270449, Abcam) and lysosome-associated membrane protein 1 (LAMP1, Cat# 99437, Cell Signaling Technology). ..

    Article Title: TJ0113-induced mitophagy in acute liver failure detected by Raman microspectroscopy.
    Article Snippet: .. Frozen liver tissue sections were fixed with 4 % polyformaldehyde (PFA), permeabilized with 0.3 % Triton X-100 for 20 min, and then blocked with 5 % BSA for 1 h. The primary antibodies used in this study included: MCL1 apoptosis regulator (Mcl-1, Cat# ab32087, Abcam), microtubule associated Protein 1 Light Chain 3 Alpha/Beta (LC3A/B, Cat# 4108, Cell Signaling Technology), mitochondrial outer membrane 20 (TOM20, Cat# 42406, Cell Signaling Technology), NLR family pyrin domain containing 3 (NLRP3, Cat# ab270449, Abcam) and lysosomeassociated membrane protein 1 (LAMP1, Cat# 99437, Cell Signaling Technology). ..



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    Investigation of mitochondrial dysfunction and microglial activation mechanisms. (A, B) Measurement of reactive oxygen species (ROS) accumulation in cells by dihydroethidium (DHE) assay and monitoring by fluorescence microscopy (A) and statistical chart (B). (C, D) Detection of mitochondrial membrane potential (MMP) in BV2 cells using 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) staining (C) and statistical chart (D). (E, F) Performance of Western blot to measure the expression levels of autophagy markers in BV2 cells (E) and statistical chart (F). (G, H) Utilization of immunofluorescence staining to determine the expression level of microtubule-associated protein 1a/1b-light chain 3 (LC3) in BV2 cells (G) and statistical chart (H). (I, J) Analysis of subcellular distribution of p62 and mitochondria (translocase of outer mitochondrial membrane 20 (Tomm20)) in BV2 cells by confocal microscopy (I) and statistical chart of positive cells (J). All cell experiments were repeated three times, and the values are presented as mean ± standard deviation (SD). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. PBS: phosphate-buffered saline; DCA: dichloroacetate; Hpgd: hydroxyprostaglandin dehydrogenase.

    Journal: Journal of Pharmaceutical Analysis

    Article Title: Exploring the impact of the liver-intestine-brain axis on brain function in non-alcoholic fatty liver disease

    doi: 10.1016/j.jpha.2024.101077

    Figure Lengend Snippet: Investigation of mitochondrial dysfunction and microglial activation mechanisms. (A, B) Measurement of reactive oxygen species (ROS) accumulation in cells by dihydroethidium (DHE) assay and monitoring by fluorescence microscopy (A) and statistical chart (B). (C, D) Detection of mitochondrial membrane potential (MMP) in BV2 cells using 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) staining (C) and statistical chart (D). (E, F) Performance of Western blot to measure the expression levels of autophagy markers in BV2 cells (E) and statistical chart (F). (G, H) Utilization of immunofluorescence staining to determine the expression level of microtubule-associated protein 1a/1b-light chain 3 (LC3) in BV2 cells (G) and statistical chart (H). (I, J) Analysis of subcellular distribution of p62 and mitochondria (translocase of outer mitochondrial membrane 20 (Tomm20)) in BV2 cells by confocal microscopy (I) and statistical chart of positive cells (J). All cell experiments were repeated three times, and the values are presented as mean ± standard deviation (SD). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. PBS: phosphate-buffered saline; DCA: dichloroacetate; Hpgd: hydroxyprostaglandin dehydrogenase.

    Article Snippet: Subsequently, the cells or tissues were treated with the translocase of outer mitochondrial membrane 20 (Tomm20) antibody (1:100; PA5-110506, Thermo Fisher, Waltham, MA, USA) for a second time, followed by an overnight incubation at 4 °C.

    Techniques: Activation Assay, Fluorescence, Microscopy, Membrane, Staining, Western Blot, Expressing, Immunofluorescence, Confocal Microscopy, Standard Deviation, Saline